Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.
نویسندگان
چکیده
منابع مشابه
The 5'-HS4 chicken beta-globin insulator is a CTCF-dependent nuclear matrix-associated element.
The protein CTCF plays an essential role in the action of a widely distributed class of vertebrate enhancer-blocking insulators, of which the first example was found in a DNA sequence element, HS4, at the 5' end of the chicken beta-globin locus. HS4 contains a binding site for CTCF that is necessary and sufficient for insulator action. Purification of CTCF has revealed that it interacts with pr...
متن کاملChicken globin gene number.
Using complementary DNA prepared from adult chicken globin messenger RNA, we show the existence of 2-3 non-cross-hybridising globin sequences in the chicken genome, each of which is present in only one copy per haploid genome. This was done by solution hybridization to total DNA under conditions of cDNA excess. The data agreses with results of RNA-driven hybridisation of globin complementary DN...
متن کاملThe human beta-globin gene contains a downstream developmental specific enhancer.
The human beta-globin gene is part of a multigene family and is expressed specifically in adult human erythroid tissue (for review, 1). When the human beta-globin is introduced into fertilized mouse eggs, it is first activated in foetal liver and remains expressed in adult erythroid tissues. It therefore mimicks the pattern of expression of its murine counterpart. It has previously been shown i...
متن کاملMultiple sequence-specific DNA binding activities are eluted from chicken nuclei at low ionic strengths.
DNA sequence-specific binding proteins eluted from chicken erythrocyte and thymus nuclei, and fractionated as described by Emerson and Felsenfeld (19), have been investigated by filter binding and footprint analyses. The erythrocyte nuclear protein fraction specifically binds to at least two sites within the 5' flanking chromatin hypersensitive site of the chicken beta A-globin gene, and to a s...
متن کاملRepeated sequence organization and RNA transcription map of the chicken adult beta-globin gene region.
We have used adaptations of the contact hybridization method to determine the repeated sequence map of a cloned fragment of chicken genomic DNA and to establish the map of RNA transcription in vivo from this same fragment. The cloned 6.2-kilobase pair (kbp) Eco RI fragment contains the adult beta-globin gene and a portion of the embryonic epsilon-globin gene. The technique of contact hybridizat...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1984
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.81.2.304